Resolution


A microscope does not deliver a perfect picture of the biological structure. Due to the law of diffraction, we observe a blurred version of it. If part of the structure is too close we will not be able resolve its detail. The so called resolution limit is a distance below which the microscope can’t resolve adjacent structures. The final effective resolution depends on a combination of the width of the PSF, signal to background, signal to noise, and sample preparation.


Prerequisites

Before starting this lesson, you should be familiar with:

Learning Objectives

After completing this lesson, learners should be able to:

  • Understand how resolution is affected by the size of the PSF

  • Understand how physical parameters of the microscope affect the resolution

Concept map

graph TD T1("TODO1") --> T2("TODO2") T2 --> T3("TODO3")

Figure


TODO



Activities

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Assessment

Fill in the blanks

  1. TODO ___ .
  2. TODO ___ .

Solution

  1. TODO
  2. TODO




Follow-up material

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