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Code of Conduct
Setup
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Tool installation
Digital image basics
Confocal microscopy image formation
Lookup tables
Spatial calibration
N-dimensional images
Data types
Image file formats
Big image data file formats
Volume subsetting and slicing
Projections
Volume rendering
Segmentation
Thresholding
Automatic thresholding (histogram-based)
Connected component labeling
Object shape measurements
Nuclei segmentation and shape measurement
Object intensity measurements
Global background correction
Neighborhood filters
Median filter
Morphological filters
Local background correction
Object filtering
2D noisy object segmentation and filtering
Distance transform
Watershed
Skeletonization
Nuclei and cells segmentation
Segment Golgi objects per cell
Manual segmentation
Deep learning instance segmentation
Setting up a scripting environment
Running a script
Recording a script
Variables
Loops
Commenting
Working with strings
Output saving
Batch processing
Functions
Handling script parameters
OME-Zarr
Correlative image rendering
Module overview
Extras
Reference
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