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Volume slicing
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Segmentation
Thresholding
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Connected component labeling
Object shape measurements
Nuclei segmentation and shape measurement
Fluorescence microscopy image formation
Object intensity measurements
Global background correction
Neighborhood filters
Median filter
2D noisy object segmentation and filtering
Morphological filters
Local background correction
Object filtering
Distance transform
Watershed
Nuclei and cells segmentation
Skeletonization
Similarity transformations
OME-Zarr
Running a script
Coding with LLMs
Recording a script
Variables
Working with strings
Output saving
Batch processing
Handling script parameters
Commenting
Setting up a scripting environment
Functions
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Correlative image rendering
Deep learning instance segmentation
Manual segmentation
Segment Golgi objects per cell
Big image data file formats
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