Difference of Gaussian

Learning Objectives

After completing this lesson, learners should be able to:

Motivation

Concept map


Figure




Difference of Gaussian (DoG)

Activity: Enhance spots in noisy image with uneven background

  • Open image: xy_8bit__two_spots_noisy_uneven_background.tif
  • Appreciate that you cannot readily threshold the spots
  • Compute DoG: - Copy image and blur with a Gaussian of small sigma -> Gs - Copy image and blur with a Gaussian of bigger sigma -> Gb - For the official DoG: rb = sqrt(2) * rs - Create DoG = Gs - Gb
  • Appreciate that now it is possible to threshold the spots in the DoG image

Learn more

  • https://imagescience.org/meijering/software/featurej/
  • https://en.wikipedia.org/wiki/Difference_of_Gaussians
  • https://github.com/CellProfiler/CellProfiler/blob/master/cellprofiler/modules/enhanceorsuppressfeatures.py#L4










Follow-up material

Recommended follow-up modules:

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